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　　Nanospray is an essential tool in high-sensitivity mass spectrometry, but limited robustness, reproducibility, and ease-of-use have historically challenged the adoption of nanospray in quantitative applications. Recent trends toward MS-based biomarker quantitation have placed strict requirements on the analytical performance of nanobore LC-MS. Nanospray MS and nanobore LC-MS both rely heavily on nanospray source hardware for successful experiments. Nanospray source hardware has matured over the past ten years from simple homemade devices to sophisticated, application-specific instrumentation featuring stage automation, thermal control, and high-resolution imaging. Many of these enhanced features provide robustness (automated tip rinsing, automated emitter change), throughput (multi-channel workflows), ease of use (multi-chip systems) or experimental flexibility. Here we present a novel nanospray source solution which delivers enhanced features of stage automation, multi-channel operation and thermal control. Ease of use has been realized through the incorporation of an integrated nanobore LC-MS consumable (PicoChip®), on which over 400 replicate injections were collected with no loss of chromatographic performance. A four channel, three column version of the source (The PicoSlide™) enables an MS-duty cycle-time of 95%, compared to 40% for a single channel system.

點(diǎn)擊下載：突破NanoLC-MS分析的通量